Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2794503..2795157 Replicon chromosome
Accession NZ_CP027457
Organism Escherichia coli strain 88-3493

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag C6W79_RS13980 Protein ID WP_000244781.1
Coordinates 2794750..2795157 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6W79_RS13975 Protein ID WP_000354046.1
Coordinates 2794503..2794769 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W79_RS13955 2790591..2792024 - 1434 WP_001613768.1 6-phospho-beta-glucosidase BglA -
C6W79_RS13960 2792069..2792380 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
C6W79_RS13965 2792544..2793203 + 660 WP_000250274.1 hemolysin III family protein -
C6W79_RS13970 2793280..2794260 - 981 WP_001613766.1 tRNA-modifying protein YgfZ -
C6W79_RS13975 2794503..2794769 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6W79_RS13980 2794750..2795157 + 408 WP_000244781.1 protein YgfX Toxin
C6W79_RS13985 2795197..2795718 - 522 WP_001613762.1 flavodoxin FldB -
C6W79_RS13990 2795830..2796726 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6W79_RS13995 2796751..2797461 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6W79_RS14000 2797467..2799200 + 1734 WP_000813202.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T99003 WP_000244781.1 NZ_CP027457:2794750-2795157 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T99003 NZ_CP027457:2794750-2795157 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT99003 WP_000354046.1 NZ_CP027457:2794503-2794769 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT99003 NZ_CP027457:2794503-2794769 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References