Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 15629..16272 Replicon plasmid unnamed2
Accession NZ_CP027456
Organism Escherichia coli strain 2014C-4423

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag C6W74_RS29055 Protein ID WP_001044768.1
Coordinates 15856..16272 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag C6W74_RS29050 Protein ID WP_001261287.1
Coordinates 15629..15859 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W74_RS29005 11493..12933 + 1441 Protein_7 enterohemolysin T1SS ABC transporter subunit EhxD -
C6W74_RS29010 13000..13143 + 144 Protein_8 transcriptional regulator -
C6W74_RS29015 13147..13338 + 192 Protein_9 lipid II-degrading bacteriocin -
C6W74_RS29020 13346..13699 - 354 WP_000864810.1 colicin M immunity protein -
C6W74_RS29025 13872..14654 - 783 WP_001164205.1 site-specific integrase -
C6W74_RS29030 14656..15069 - 414 WP_000465041.1 hypothetical protein -
C6W74_RS29050 15629..15859 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C6W74_RS29055 15856..16272 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
C6W74_RS29070 16775..17987 - 1213 Protein_15 IS3 family transposase -
C6W74_RS29075 18043..20292 - 2250 Protein_16 hypothetical protein -
C6W74_RS29080 20310..20693 - 384 WP_101329730.1 hydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyC / hlyA / hlyB / hlyD / hlyD / espP / toxB 1..79682 79682


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T98997 WP_001044768.1 NZ_CP027456:15856-16272 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T98997 NZ_CP027456:15856-16272 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT98997 WP_001261287.1 NZ_CP027456:15629-15859 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT98997 NZ_CP027456:15629-15859 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References