Detailed information of TA system TA098992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 43965..44524
• Replicon: plasmid unnamed1
• Accession: NZ_CP027455
• Organism: Escherichia coli strain 2014C-4423

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: C6W74_RS28790
• Protein ID: WP_000421257.1
• Coordinates: 43965..44240 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C6W74_RS28795
• Protein ID: WP_024223171.1
• Coordinates: 44240..44524 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W74_RS28745	39149..39424	+	276	WP_000239529.1	hypothetical protein	-
C6W74_RS28750	39562..40341	-	780	WP_001302707.1	site-specific integrase	-
C6W74_RS28755	40392..41171	-	780	WP_000535296.1	hypothetical protein	-
C6W74_RS28760	41224..41538	-	315	WP_001302628.1	hypothetical protein	-
C6W74_RS28765	42109..42360	+	252	WP_021553173.1	hypothetical protein	-
C6W74_RS28770	42360..42950	+	591	WP_021553172.1	hypothetical protein	-
C6W74_RS28775	42968..43315	-	348	WP_000142451.1	hypothetical protein	-
C6W74_RS28785	43667..43858	-	192	WP_021553171.1	hypothetical protein	-
C6W74_RS28790	43965..44240	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6W74_RS28795	44240..44524	-	285	WP_024223171.1	ribbon-helix-helix domain-containing protein	Antitoxin
C6W74_RS28810	45434..46291	-	858	WP_021569951.1	incFII family plasmid replication initiator RepA	-
C6W74_RS28815	46284..46358	-	75	WP_001365705.1	RepA leader peptide Tap	-
C6W74_RS28825	46592..46849	-	258	WP_021553168.1	replication regulatory protein RepA	-
C6W74_RS28830	47133..47282	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	-
-	47327..47386	+	60	NuclAT_1	-	-
-	47327..47386	+	60	NuclAT_1	-	-
-	47327..47386	+	60	NuclAT_1	-	-
-	47327..47386	+	60	NuclAT_1	-	-
C6W74_RS28840	47529..48002	-	474	WP_021553167.1	hypothetical protein	-
C6W74_RS28845	48157..48750	-	594	WP_021553166.1	DUF2726 domain-containing protein	-
C6W74_RS28850	48788..48997	-	210	WP_001333231.1	hemolysin expression modulator Hha	-
C6W74_RS28855	49043..49504	-	462	WP_021553165.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..73262	73262

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T98992 WP_000421257.1 NZ_CP027455:c44240-43965 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T98992 NZ_CP027455:c44240-43965 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTACTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10504.92 Da
• Isoelectric Point: 5.6558

>AT98992 WP_024223171.1 NZ_CP027455:c44524-44240 [Escherichia coli]
MQMKNNTTQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT98992 NZ_CP027455:c44524-44240 [Escherichia coli]
ATGCAAATGAAAAACAATACCACACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5