Detailed information of TA system TA098985

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5188608..5189445
• Replicon: chromosome
• Accession: NZ_CP027454
• Organism: Escherichia coli strain 2014C-4423

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: C6W74_RS27505
• Protein ID: WP_000854687.1
• Coordinates: 5188608..5188988 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LJJ3
• Locus tag: C6W74_RS27510
• Protein ID: WP_001443392.1
• Coordinates: 5189077..5189445 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W74_RS27475	5184250..5186091	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
C6W74_RS27480	5186170..5186676	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
C6W74_RS27490	5186976..5187821	-	846	WP_001274557.1	DUF4942 domain-containing protein	-
C6W74_RS27495	5187906..5188103	-	198	WP_000772026.1	DUF957 domain-containing protein	-
C6W74_RS27500	5188123..5188611	-	489	WP_001054228.1	hypothetical protein	-
C6W74_RS27505	5188608..5188988	-	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
C6W74_RS27510	5189077..5189445	-	369	WP_001443392.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6W74_RS27515	5189521..5189742	-	222	WP_000692312.1	DUF987 domain-containing protein	-
C6W74_RS27520	5189805..5190281	-	477	WP_001186728.1	RadC family protein	-
C6W74_RS27525	5190297..5190770	-	474	WP_000855068.1	antirestriction protein	-
C6W74_RS27530	5190864..5191109	-	246	WP_001164974.1	hypothetical protein	-
C6W74_RS27535	5191109..5191549	-	441	Protein_5040	DUF932 domain-containing protein	-
C6W74_RS27540	5191668..5193446	-	1779	WP_001243192.1	reverse transcriptase/maturase family protein	-
C6W74_RS27545	5193956..5194339	-	384	Protein_5042	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T98985 WP_000854687.1 NZ_CP027454:c5188988-5188608 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T98985 NZ_CP027454:c5188988-5188608 [Escherichia coli]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13781.54 Da
• Isoelectric Point: 6.2049

>AT98985 WP_001443392.1 NZ_CP027454:c5189445-5189077 [Escherichia coli]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT98985 NZ_CP027454:c5189445-5189077 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67

Antitoxin

• AlphaFold DB: A0A7U9LJJ3