Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 5015527..5016325 | Replicon | chromosome |
| Accession | NZ_CP027454 | ||
| Organism | Escherichia coli strain 2014C-4423 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A3W5Y7S6 |
| Locus tag | C6W74_RS26510 | Protein ID | WP_000854916.1 |
| Coordinates | 5015527..5015904 (-) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A3W5Y7S1 |
| Locus tag | C6W74_RS26515 | Protein ID | WP_024174207.1 |
| Coordinates | 5015951..5016325 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C6W74_RS26485 | 5011978..5012652 | + | 675 | WP_106901533.1 | IS66-like element accessory protein TnpA | - |
| C6W74_RS26490 | 5012649..5012996 | + | 348 | WP_000631725.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| C6W74_RS26495 | 5013016..5014572 | + | 1557 | WP_001066419.1 | IS66 family transposase | - |
| C6W74_RS30020 | 5014590..5014748 | - | 159 | WP_000014512.1 | hypothetical protein | - |
| C6W74_RS26500 | 5014833..5015030 | - | 198 | WP_000839246.1 | DUF957 domain-containing protein | - |
| C6W74_RS26505 | 5015042..5015530 | - | 489 | WP_000777666.1 | hypothetical protein | - |
| C6W74_RS26510 | 5015527..5015904 | - | 378 | WP_000854916.1 | TA system toxin CbtA family protein | Toxin |
| C6W74_RS26515 | 5015951..5016325 | - | 375 | WP_024174207.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| C6W74_RS26520 | 5016488..5016709 | - | 222 | WP_000692312.1 | DUF987 domain-containing protein | - |
| C6W74_RS26525 | 5016778..5016912 | - | 135 | Protein_4854 | hypothetical protein | - |
| C6W74_RS26530 | 5016953..5018150 | + | 1198 | Protein_4855 | IS3 family transposase | - |
| C6W74_RS26535 | 5018228..5018578 | - | 351 | WP_000624681.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| C6W74_RS26540 | 5018575..5019000 | - | 426 | WP_000422707.1 | IS66 family insertion sequence hypothetical protein | - |
| C6W74_RS26545 | 5019132..5019485 | - | 354 | Protein_4858 | transposase | - |
| C6W74_RS26550 | 5019523..5020736 | + | 1214 | WP_101329727.1 | IS3 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | espK / espF / escG / escF / cesD2 / espB / espD / espA / sepL / escD / eae / cesT / tir / map / cesF / espH / sepQ/escQ / escO / escN / escV / cesL / escI / escJ / sepD / escC / cesD / etgA / escU / escT / escS / escR / escL / escK / cesAB / escE / espG / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD | 5002744..5077409 | 74665 | |
| - | inside | IScluster/Tn | - | - | 5010331..5020736 | 10405 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14033.04 Da Isoelectric Point: 9.1378
>T98983 WP_000854916.1 NZ_CP027454:c5015904-5015527 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIKAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITPGKHPEAKQ
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIKAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITPGKHPEAKQ
Download Length: 378 bp
>T98983 NZ_CP027454:c5015904-5015527 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTA
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCCGGGGAAGCATCCGGAGGCGAAACAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTA
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCCGGGGAAGCATCCGGAGGCGAAACAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13789.59 Da Isoelectric Point: 6.7387
>AT98983 WP_024174207.1 NZ_CP027454:c5016325-5015951 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTIYPTPATATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTIYPTPATATTS
Download Length: 375 bp
>AT98983 NZ_CP027454:c5016325-5015951 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTATTTATCCGACACCGGCAACCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTATTTATCCGACACCGGCAACCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3W5Y7S6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3W5Y7S1 |