Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4930701..4931361 Replicon chromosome
Accession NZ_CP027454
Organism Escherichia coli strain 2014C-4423

Toxin (Protein)


Gene name cptA Uniprot ID A0A3W5XVE6
Locus tag C6W74_RS26035 Protein ID WP_000244778.1
Coordinates 4930701..4931114 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6W74_RS26040 Protein ID WP_000354046.1
Coordinates 4931095..4931361 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W74_RS26015 4926665..4928398 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
C6W74_RS26020 4928404..4929114 - 711 WP_000715231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6W74_RS26025 4929138..4930034 - 897 WP_032209841.1 site-specific tyrosine recombinase XerD -
C6W74_RS26030 4930146..4930667 + 522 WP_001055874.1 flavodoxin FldB -
C6W74_RS26035 4930701..4931114 - 414 WP_000244778.1 protein YgfX Toxin
C6W74_RS26040 4931095..4931361 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6W74_RS26045 4931604..4932584 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C6W74_RS26050 4932661..4933320 - 660 WP_000250274.1 hemolysin III family protein -
C6W74_RS26055 4933484..4933795 - 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
C6W74_RS26060 4933840..4935273 + 1434 WP_001343615.1 6-phospho-beta-glucosidase BglA -
C6W74_RS26065 4935330..4936073 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16275.23 Da        Isoelectric Point: 11.7064

>T98982 WP_000244778.1 NZ_CP027454:c4931114-4930701 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Download         Length: 414 bp

>T98982 NZ_CP027454:c4931114-4930701 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT98982 WP_000354046.1 NZ_CP027454:c4931361-4931095 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT98982 NZ_CP027454:c4931361-4931095 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5XVE6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References