Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3253658..3254296 Replicon chromosome
Accession NZ_CP027454
Organism Escherichia coli strain 2014C-4423

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C6W74_RS17215 Protein ID WP_000813794.1
Coordinates 3254120..3254296 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C6W74_RS17210 Protein ID WP_001270286.1
Coordinates 3253658..3254074 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W74_RS17190 3248810..3249751 - 942 WP_001251313.1 ABC transporter permease -
C6W74_RS17195 3249752..3250765 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C6W74_RS17200 3250783..3251928 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
C6W74_RS17205 3252173..3253579 - 1407 WP_000760614.1 PLP-dependent aminotransferase family protein -
C6W74_RS17210 3253658..3254074 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C6W74_RS17215 3254120..3254296 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C6W74_RS17220 3254518..3254748 + 231 WP_000494244.1 YncJ family protein -
C6W74_RS17225 3254840..3256801 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C6W74_RS17230 3256874..3257410 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
C6W74_RS17235 3257463..3258677 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T98969 WP_000813794.1 NZ_CP027454:c3254296-3254120 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T98969 NZ_CP027454:c3254296-3254120 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT98969 WP_001270286.1 NZ_CP027454:c3254074-3253658 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT98969 NZ_CP027454:c3254074-3253658 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCACCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References