Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2728771..2729242 Replicon chromosome
Accession NZ_CP027454
Organism Escherichia coli strain 2014C-4423

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C6W74_RS14190 Protein ID WP_001303511.1
Coordinates 2728771..2729049 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C6W74_RS14195 Protein ID WP_001302048.1
Coordinates 2729051..2729242 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W74_RS14160 2724259..2724528 - 270 WP_000003742.1 excisionase -
C6W74_RS14165 2724590..2727061 - 2472 WP_000048560.1 exonuclease -
C6W74_RS14170 2727185..2727406 - 222 WP_000560212.1 cell division protein FtsZ -
C6W74_RS14180 2728119..2728418 - 300 WP_001240334.1 hypothetical protein -
C6W74_RS14190 2728771..2729049 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6W74_RS14195 2729051..2729242 - 192 WP_001302048.1 hypothetical protein Antitoxin
C6W74_RS14200 2729263..2729634 - 372 WP_001169687.1 hypothetical protein -
C6W74_RS14205 2729732..2730034 + 303 WP_000172738.1 transcriptional regulator -
C6W74_RS14210 2730031..2730456 + 426 WP_000693944.1 Rha family transcriptional regulator -
C6W74_RS14215 2730479..2731441 + 963 WP_000095671.1 helix-turn-helix domain-containing protein -
C6W74_RS14220 2731482..2731904 + 423 WP_001151187.1 DUF977 family protein -
C6W74_RS14225 2732010..2732222 + 213 WP_000935423.1 hypothetical protein -
C6W74_RS14230 2732255..2732473 + 219 WP_000209148.1 DUF4014 family protein -
C6W74_RS29570 2732475..2732633 + 159 WP_001370098.1 hypothetical protein -
C6W74_RS14235 2732637..2734193 - 1557 WP_001066422.1 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2716423..2767053 50630


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T98964 WP_001303511.1 NZ_CP027454:c2729049-2728771 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T98964 NZ_CP027454:c2729049-2728771 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98964 WP_001302048.1 NZ_CP027454:c2729242-2729051 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98964 NZ_CP027454:c2729242-2729051 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References