Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1110830..1111327 Replicon chromosome
Accession NZ_CP027454
Organism Escherichia coli strain 2014C-4423

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C6W74_RS05790 Protein ID WP_001173343.1
Coordinates 1111154..1111327 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A3W5Y3H9
Locus tag C6W74_RS05785 Protein ID WP_071532590.1
Coordinates 1110830..1111126 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W74_RS05760 1107561..1108280 - 720 WP_000611288.1 two-component system response regulator DcuR -
C6W74_RS05765 1108277..1109908 - 1632 WP_001216468.1 sensor histidine kinase -
C6W74_RS05770 1110089..1110319 + 231 WP_000371698.1 (4Fe-4S)-binding protein -
C6W74_RS05775 1110331..1110603 + 273 WP_000405647.1 N-acetyltransferase -
C6W74_RS05785 1110830..1111126 + 297 WP_071532590.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C6W74_RS05790 1111154..1111327 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C6W74_RS05795 1111446..1112963 - 1518 WP_001295082.1 lysine--tRNA ligase -
C6W74_RS05800 1113200..1114657 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(7-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T98959 WP_001173343.1 NZ_CP027454:1111154-1111327 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T98959 NZ_CP027454:1111154-1111327 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11482.04 Da        Isoelectric Point: 4.2931

>AT98959 WP_071532590.1 NZ_CP027454:1110830-1111126 [Escherichia coli]
MEGKKKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT98959 NZ_CP027454:1110830-1111126 [Escherichia coli]
ATGGAAGGTAAAAAAAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A3W5Y3H9

References