Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 317131..317762 Replicon chromosome
Accession NZ_CP027454
Organism Escherichia coli strain 2014C-4423

Toxin (Protein)


Gene name hicA Uniprot ID A0A377MSE5
Locus tag C6W74_RS01750 Protein ID WP_001259383.1
Coordinates 317487..317762 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6W74_RS01745 Protein ID WP_000593555.1
Coordinates 317131..317490 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W74_RS01720 313263..314207 + 945 WP_000947089.1 nickel ABC transporter permease subunit NikB -
C6W74_RS01725 314204..315037 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
C6W74_RS01730 315037..315801 + 765 WP_001136244.1 nickel import ATP-binding protein NikD -
C6W74_RS01735 315798..316604 + 807 WP_000173673.1 nickel import ATP-binding protein NikE -
C6W74_RS01740 316610..317011 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C6W74_RS01745 317131..317490 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6W74_RS01750 317487..317762 - 276 WP_001259383.1 type II toxin-antitoxin system HicA family toxin Toxin
C6W74_RS01755 317835..318959 - 1125 WP_001343434.1 ABC transporter permease -
C6W74_RS01760 318959..321694 - 2736 WP_000149120.1 ribosome-associated ATPase/putative transporter RbbA -
C6W74_RS01765 321691..322758 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10209.98 Da        Isoelectric Point: 10.7076

>T98956 WP_001259383.1 NZ_CP027454:c317762-317487 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGPRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T98956 NZ_CP027454:c317762-317487 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTCCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT98956 WP_000593555.1 NZ_CP027454:c317490-317131 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT98956 NZ_CP027454:c317490-317131 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377MSE5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References