Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 19271..19796 Replicon plasmid unnamed
Accession NZ_CP027453
Organism Escherichia coli strain 2014C-3338

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C6W71_RS24950 Protein ID WP_001159871.1
Coordinates 19271..19576 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C6W71_RS24955 Protein ID WP_000813639.1
Coordinates 19578..19796 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W71_RS24920 15266..15439 + 174 Protein_18 DUF4113 domain-containing protein -
C6W71_RS24925 15441..15857 - 417 WP_001278809.1 plasmid partitioning/stability family protein -
C6W71_RS24930 15850..16830 - 981 WP_000688510.1 plasmid segregation protein ParM -
C6W71_RS24935 17245..17553 - 309 WP_000030199.1 hypothetical protein -
C6W71_RS24940 17640..18284 - 645 WP_001144036.1 ParA family protein -
C6W71_RS24945 18464..19270 - 807 WP_000016966.1 site-specific integrase -
C6W71_RS24950 19271..19576 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C6W71_RS24955 19578..19796 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6W71_RS24960 20392..20652 + 261 WP_000343100.1 hypothetical protein -
C6W71_RS24965 20649..21239 + 591 WP_033802802.1 hypothetical protein -
C6W71_RS24970 21257..21604 - 348 WP_000142427.1 hypothetical protein -
C6W71_RS24980 22074..22409 - 336 Protein_29 colicin -
C6W71_RS24985 22504..23235 - 732 WP_000850413.1 complement resistance protein TraT -
C6W71_RS26105 23512..23622 - 111 Protein_31 IS481 family transposase -
C6W71_RS26110 23626..23837 - 212 Protein_32 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyD / hlyB / hlyA / hlyC / espP 1..159611 159611


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T98955 WP_001159871.1 NZ_CP027453:c19576-19271 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T98955 NZ_CP027453:c19576-19271 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT98955 WP_000813639.1 NZ_CP027453:c19796-19578 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT98955 NZ_CP027453:c19796-19578 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References