Detailed information of TA system TA098954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4492742..4493378
• Replicon: chromosome
• Accession: NZ_CP027452
• Organism: Escherichia coli strain 2014C-3338

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: C6W71_RS23105
• Protein ID: WP_000813796.1
• Coordinates: 4493202..4493378 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C6W71_RS23100
• Protein ID: WP_076838470.1
• Coordinates: 4492742..4493158 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W71_RS23080	4487894..4488835	-	942	WP_001251307.1	ABC transporter permease	-
C6W71_RS23085	4488836..4489849	-	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
C6W71_RS23090	4489867..4491012	-	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
C6W71_RS23095	4491257..4492663	-	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
C6W71_RS23100	4492742..4493158	-	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C6W71_RS23105	4493202..4493378	-	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C6W71_RS23110	4493600..4493830	+	231	WP_000494243.1	YncJ family protein	-
C6W71_RS23115	4493922..4495883	-	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C6W71_RS23120	4495956..4496492	-	537	WP_106889543.1	DNA-binding transcriptional regulator SutR	-
C6W71_RS23125	4496545..4497756	+	1212	WP_071593090.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T98954 WP_000813796.1 NZ_CP027452:c4493378-4493202 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T98954 NZ_CP027452:c4493378-4493202 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT98954 WP_076838470.1 NZ_CP027452:c4493158-4492742 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT98954 NZ_CP027452:c4493158-4492742 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3