Detailed information of TA system TA098940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2385213..2385808
• Replicon: chromosome
• Accession: NZ_CP027452
• Organism: Escherichia coli strain 2014C-3338

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D6JG31
• Locus tag: C6W71_RS12295
• Protein ID: WP_000155159.1
• Coordinates: 2385431..2385808 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: C6W71_RS12290
• Protein ID: WP_000557315.1
• Coordinates: 2385213..2385434 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W71_RS12265	2380289..2381398	+	1110	WP_001318085.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
C6W71_RS12270	2381446..2382372	+	927	WP_000003010.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
C6W71_RS12275	2382369..2383646	+	1278	WP_000803825.1	branched chain amino acid ABC transporter permease LivM	-
C6W71_RS12280	2383643..2384410	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
C6W71_RS12285	2384412..2385125	+	714	WP_106889442.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
C6W71_RS12290	2385213..2385434	+	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C6W71_RS12295	2385431..2385808	+	378	WP_000155159.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C6W71_RS12300	2386017..2387333	+	1317	WP_000803191.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
C6W71_RS12305	2387509..2388396	+	888	WP_000099289.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
C6W71_RS12310	2388393..2389238	+	846	WP_000572164.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
C6W71_RS12315	2389240..2390310	+	1071	WP_000907828.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2375101..2391050	15949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.25 Da
• Isoelectric Point: 6.7263

>T98940 WP_000155159.1 NZ_CP027452:2385431-2385808 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T98940 NZ_CP027452:2385431-2385808 [Escherichia coli]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGTTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT98940 WP_000557315.1 NZ_CP027452:2385213-2385434 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT98940 NZ_CP027452:2385213-2385434 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTAGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATCAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: D6JG31

Antitoxin

• AlphaFold DB: D6JG32