Detailed information of TA system TA098939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2357937..2358568
• Replicon: chromosome
• Accession: NZ_CP027452
• Organism: Escherichia coli strain 2014C-3338

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: C6W71_RS12135
• Protein ID: WP_001260301.1
• Coordinates: 2357937..2358212 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: C6W71_RS12140
• Protein ID: WP_000593557.1
• Coordinates: 2358209..2358568 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W71_RS12120	2352941..2354008	+	1068	WP_000361470.1	HlyD family secretion protein	-
C6W71_RS12125	2354005..2356740	+	2736	WP_052920229.1	ribosome-associated ATPase/putative transporter RbbA	-
C6W71_RS12130	2356740..2357864	+	1125	WP_001318088.1	ABC transporter permease	-
C6W71_RS12135	2357937..2358212	+	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
C6W71_RS12140	2358209..2358568	+	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C6W71_RS12145	2358688..2359089	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
C6W71_RS12150	2359095..2359901	-	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
C6W71_RS12155	2359898..2360662	-	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
C6W71_RS12160	2360662..2361495	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
C6W71_RS12165	2361492..2362436	-	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T98939 WP_001260301.1 NZ_CP027452:2357937-2358212 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T98939 NZ_CP027452:2357937-2358212 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT98939 WP_000593557.1 NZ_CP027452:2358209-2358568 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT98939 NZ_CP027452:2358209-2358568 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1