Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1531432..1531929 Replicon chromosome
Accession NZ_CP027452
Organism Escherichia coli strain 2014C-3338

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C6W71_RS07950 Protein ID WP_001173343.1
Coordinates 1531432..1531605 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag C6W71_RS07955 Protein ID WP_001317561.1
Coordinates 1531633..1531929 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W71_RS07940 1528102..1529559 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
C6W71_RS07945 1529796..1531313 + 1518 WP_000003805.1 lysine--tRNA ligase -
C6W71_RS07950 1531432..1531605 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C6W71_RS07955 1531633..1531929 - 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C6W71_RS07965 1532156..1532429 - 274 Protein_1457 N-acetyltransferase -
C6W71_RS07970 1532441..1532671 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
C6W71_RS07975 1532852..1534483 + 1632 WP_001216482.1 sensor histidine kinase -
C6W71_RS07980 1534480..1535199 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T98932 WP_001173343.1 NZ_CP027452:c1531605-1531432 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T98932 NZ_CP027452:c1531605-1531432 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT98932 WP_001317561.1 NZ_CP027452:c1531929-1531633 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT98932 NZ_CP027452:c1531929-1531633 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References