Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 757771..758389 Replicon chromosome
Accession NZ_CP027452
Organism Escherichia coli strain 2014C-3338

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C6W71_RS04070 Protein ID WP_001291435.1
Coordinates 758171..758389 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C6W71_RS04065 Protein ID WP_000344800.1
Coordinates 757771..758145 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W71_RS04055 752860..754053 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C6W71_RS04060 754076..757225 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
C6W71_RS04065 757771..758145 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C6W71_RS04070 758171..758389 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C6W71_RS04075 758561..759112 + 552 WP_000102564.1 maltose O-acetyltransferase -
C6W71_RS04080 759228..759698 + 471 WP_000136192.1 YlaC family protein -
C6W71_RS04085 759862..761412 + 1551 WP_042104058.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C6W71_RS04090 761454..761807 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C6W71_RS04100 762186..762497 + 312 WP_042104056.1 MGMT family protein -
C6W71_RS04105 762528..763100 - 573 WP_032237088.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T98927 WP_001291435.1 NZ_CP027452:758171-758389 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T98927 NZ_CP027452:758171-758389 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT98927 WP_000344800.1 NZ_CP027452:757771-758145 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT98927 NZ_CP027452:757771-758145 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References