Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/Xre(antitoxin)
Location 19259..19757 Replicon plasmid unnamed1
Accession NZ_CP027450
Organism Escherichia coli strain 2014C-3097

Toxin (Protein)


Gene name TsxA Uniprot ID A0A7L6LHZ8
Locus tag C6W73_RS26535 Protein ID WP_000868277.1
Coordinates 19491..19757 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID A0A3H7VMJ5
Locus tag C6W73_RS26530 Protein ID WP_001450803.1
Coordinates 19259..19501 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W73_RS26485 14483..14818 - 336 WP_000963832.1 hypothetical protein -
C6W73_RS26490 15164..15571 + 408 WP_001190621.1 hypothetical protein -
C6W73_RS26495 15581..16342 + 762 WP_001097938.1 conjugal transfer protein TraL -
C6W73_RS26500 16339..17044 + 706 Protein_14 hypothetical protein -
C6W73_RS26505 17073..17510 - 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin -
C6W73_RS26510 17535..17717 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin -
C6W73_RS26520 18489..18818 - 330 WP_021558913.1 hypothetical protein -
C6W73_RS26530 19259..19501 - 243 WP_001450803.1 hypothetical protein Antitoxin
C6W73_RS26535 19491..19757 - 267 WP_000868277.1 hypothetical protein Toxin
C6W73_RS26545 20129..20353 - 225 WP_064565522.1 hypothetical protein -
C6W73_RS26550 20396..20719 - 324 WP_064565519.1 hypothetical protein -
C6W73_RS26560 22326..23339 + 1014 WP_024176825.1 replication initiation protein -
C6W73_RS26565 23463..23732 + 270 WP_000190069.1 hypothetical protein -
C6W73_RS26570 23995..24267 + 273 WP_074400780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..34867 34867


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10205.74 Da        Isoelectric Point: 10.4299

>T98922 WP_000868277.1 NZ_CP027450:c19757-19491 [Escherichia coli]
MKVRKGDRQYYLNKEGNMFHLVKRVKTFSKSATLGKTKATVKTVADLVFNEKAFDTIDFSRDGLRENDKEIVSMMIQEMN
NERGKNVN

Download         Length: 267 bp

>T98922 NZ_CP027450:c19757-19491 [Escherichia coli]
ATGAAGGTACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTAATATGTTTCATCTTGTGAAACGGGTGAAGAC
CTTTTCTAAATCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTAATGAGAAGGCAT
TCGACACAATAGACTTTTCCAGAGATGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGAGGAAAAAATGTTAACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9288.69 Da        Isoelectric Point: 9.7120

>AT98922 WP_001450803.1 NZ_CP027450:c19501-19259 [Escherichia coli]
MLTENTPENIKRLRQKIGLTQRECAEIFSMNPRTWRRKEEPAGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Download         Length: 243 bp

>AT98922 NZ_CP027450:c19501-19259 [Escherichia coli]
ATGTTAACTGAAAACACACCAGAAAACATCAAACGTCTGCGGCAAAAAATTGGCCTGACGCAAAGAGAATGTGCTGAAAT
ATTCAGCATGAACCCGCGAACCTGGCGTCGCAAGGAAGAACCTGCCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTCCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHZ8

Antitoxin

Source ID Structure
AlphaFold DB A0A3H7VMJ5

References