Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 17073..17717 Replicon plasmid unnamed1
Accession NZ_CP027450
Organism Escherichia coli strain 2014C-3097

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag C6W73_RS26510 Protein ID WP_000833471.1
Coordinates 17535..17717 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag C6W73_RS26505 Protein ID WP_000466317.1
Coordinates 17073..17510 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W73_RS26485 14483..14818 - 336 WP_000963832.1 hypothetical protein -
C6W73_RS26490 15164..15571 + 408 WP_001190621.1 hypothetical protein -
C6W73_RS26495 15581..16342 + 762 WP_001097938.1 conjugal transfer protein TraL -
C6W73_RS26500 16339..17044 + 706 Protein_14 hypothetical protein -
C6W73_RS26505 17073..17510 - 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6W73_RS26510 17535..17717 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
C6W73_RS26520 18489..18818 - 330 WP_021558913.1 hypothetical protein -
C6W73_RS26530 19259..19501 - 243 WP_001450803.1 hypothetical protein -
C6W73_RS26535 19491..19757 - 267 WP_000868277.1 hypothetical protein -
C6W73_RS26545 20129..20353 - 225 WP_064565522.1 hypothetical protein -
C6W73_RS26550 20396..20719 - 324 WP_064565519.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..34867 34867


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T98921 WP_000833471.1 NZ_CP027450:c17717-17535 [Escherichia coli]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T98921 NZ_CP027450:c17717-17535 [Escherichia coli]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT98921 WP_000466317.1 NZ_CP027450:c17510-17073 [Escherichia coli]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT98921 NZ_CP027450:c17510-17073 [Escherichia coli]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAGCTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References