Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3084359..3085013 Replicon chromosome
Accession NZ_CP027449
Organism Escherichia coli strain 2014C-3097

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag C6W73_RS15910 Protein ID WP_000244772.1
Coordinates 3084606..3085013 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6W73_RS15905 Protein ID WP_000354046.1
Coordinates 3084359..3084625 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W73_RS15880 3079647..3080390 + 744 WP_001570285.1 SDR family oxidoreductase -
C6W73_RS15885 3080447..3081880 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
C6W73_RS15890 3081925..3082236 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C6W73_RS15895 3082400..3083059 + 660 WP_000250274.1 hemolysin III family protein -
C6W73_RS15900 3083136..3084116 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C6W73_RS15905 3084359..3084625 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6W73_RS15910 3084606..3085013 + 408 WP_000244772.1 protein YgfX Toxin
C6W73_RS15915 3085053..3085574 - 522 WP_001055874.1 flavodoxin FldB -
C6W73_RS15920 3085686..3086582 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6W73_RS15925 3086607..3087317 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6W73_RS15930 3087323..3089056 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T98908 WP_000244772.1 NZ_CP027449:3084606-3085013 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T98908 NZ_CP027449:3084606-3085013 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT98908 WP_000354046.1 NZ_CP027449:3084359-3084625 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT98908 NZ_CP027449:3084359-3084625 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References