Detailed information of TA system TA098892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 107155..107780
• Replicon: plasmid unnamed
• Accession: NZ_CP027448
• Organism: Escherichia coli strain 2014C-3075

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6W72_RS27025
• Protein ID: WP_106901831.1
• Coordinates: 107382..107780 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: C6W72_RS27020
• Protein ID: WP_000450532.1
• Coordinates: 107155..107382 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W72_RS27020	107155..107382	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6W72_RS27025	107382..107780	+	399	WP_106901831.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6W72_RS27030	107789..109942	-	2154	WP_106901832.1	type IV conjugative transfer system coupling protein TraD	-
C6W72_RS27035	110194..110925	-	732	WP_000850423.1	complement resistance protein TraT	-
C6W72_RS27040	110939..111448	-	510	WP_106901833.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / faeC / faeD / faeE / faeF / faeH / faeI	1..170848	170848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14856.16 Da
• Isoelectric Point: 9.2202

>T98892 WP_106901831.1 NZ_CP027448:107382-107780 [Escherichia coli]
MLKFMLNTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T98892 NZ_CP027448:107382-107780 [Escherichia coli]
ATGCTGAAGTTTATGCTCAATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT98892 WP_000450532.1 NZ_CP027448:107155-107382 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT98892 NZ_CP027448:107155-107382 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5