Detailed information of TA system TA098891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 6196..6953
• Replicon: plasmid unnamed
• Accession: NZ_CP027448
• Organism: Escherichia coli strain 2014C-3075

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A136WTZ1
• Locus tag: C6W72_RS26460
• Protein ID: WP_000501973.1
• Coordinates: 6196..6681 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: C6W72_RS26465
• Protein ID: WP_001352814.1
• Coordinates: 6669..6953 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W72_RS26435	1921..3147	+	1227	WP_057729518.1	phosphoadenosine phosphosulfate reductase	-
C6W72_RS26440	3132..3776	+	645	WP_106901802.1	ParB N-terminal domain-containing protein	-
C6W72_RS26445	3940..4143	-	204	Protein_4	transposase	-
C6W72_RS26455	4879..5901	+	1023	WP_106901803.1	IS110 family transposase	-
C6W72_RS26460	6196..6681	-	486	WP_000501973.1	GNAT family N-acetyltransferase	Toxin
C6W72_RS26465	6669..6953	-	285	WP_001352814.1	DUF1778 domain-containing protein	Antitoxin
C6W72_RS26470	7344..8831	-	1488	WP_106901861.1	alpha/beta hydrolase	-
C6W72_RS26475	8936..9883	-	948	WP_106901804.1	omptin family outer membrane protease OmpP	-
C6W72_RS26480	10121..10276	-	156	Protein_10	transposase domain-containing protein	-
C6W72_RS26485	10332..10586	-	255	Protein_11	IS3 family transposase	-
C6W72_RS26490	10697..11122	+	426	WP_106901805.1	transposase	-
C6W72_RS26495	11119..11469	+	351	WP_000624720.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / faeC / faeD / faeE / faeF / faeH / faeI	1..170848	170848
	-	inside	IScluster/Tn	-	-	4879..13113	8234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-93)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17656.44 Da
• Isoelectric Point: 9.6133

>T98891 WP_000501973.1 NZ_CP027448:c6681-6196 [Escherichia coli]
MGCVTAPEPLSSFHQVAEFVSGETVLDDWLKQKGLKNQALGATRTFVVCRKGTQQVVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDASFRGKGLGADLLHDAVCRCYRVAENIGVRAIMVHALTENAKHFYIHHGFKPSKTQVQTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T98891 NZ_CP027448:c6681-6196 [Escherichia coli]
ATGGGATGTGTAACAGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAACTGTTCTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCTCTCGGAGCCACCCGGACTTTTGTTGTATGCAGGAAAGGTACGC
AACAAGTAGTTGGTTTTTACTCACTGGCTACTGGCAGTGTCAACCATACAGAAGCTACAGGCAACCTTCGGCGCAATATG
CCTGACCCTATCCCTGTCATTATACTTGCACGCCTGGCTGTTGATGCGTCATTCCGTGGTAAAGGGCTTGGTGCAGATTT
ACTGCATGATGCGGTTTGTCGTTGTTACAGAGTGGCCGAAAATATAGGTGTGCGTGCAATTATGGTTCATGCACTTACGG
AAAACGCAAAACACTTTTATATTCACCATGGTTTTAAGCCATCAAAAACCCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10915.58 Da
• Isoelectric Point: 4.6926

>AT98891 WP_001352814.1 NZ_CP027448:c6953-6669 [Escherichia coli]
MIYGGFMKSGVQLNLRARESQRILIDTAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADEP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT98891 NZ_CP027448:c6953-6669 [Escherichia coli]
ATGATATACGGAGGTTTTATGAAATCAGGTGTTCAACTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGACAC
TGCTGCAGAAATTCTTCATAAGTCACGTACAGACTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATCGAAATGCTTGATGCTCCGGTTGCAGATGAGCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Structures

Toxin

• AlphaFold DB: A0A136WTZ1