Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4864304..4864922 Replicon chromosome
Accession NZ_CP027447
Organism Escherichia coli strain 2014C-3075

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C6W72_RS25015 Protein ID WP_001291435.1
Coordinates 4864304..4864522 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C6W72_RS25020 Protein ID WP_000344800.1
Coordinates 4864548..4864922 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W72_RS24980 4859593..4860165 + 573 WP_000779831.1 YbaY family lipoprotein -
C6W72_RS24985 4860196..4860507 - 312 WP_000409911.1 MGMT family protein -
C6W72_RS24995 4860886..4861239 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C6W72_RS25000 4861281..4862831 - 1551 WP_089583158.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C6W72_RS25005 4862995..4863465 - 471 WP_022645280.1 YlaC family protein -
C6W72_RS25010 4863581..4864132 - 552 WP_096986530.1 maltose O-acetyltransferase -
C6W72_RS25015 4864304..4864522 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C6W72_RS25020 4864548..4864922 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C6W72_RS25025 4865468..4868617 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
C6W72_RS25030 4868640..4869833 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T98889 WP_001291435.1 NZ_CP027447:c4864522-4864304 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T98889 NZ_CP027447:c4864522-4864304 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT98889 WP_000344800.1 NZ_CP027447:c4864922-4864548 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT98889 NZ_CP027447:c4864922-4864548 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACTTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTCCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References