Detailed information of TA system TA098887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4356323..4356873
• Replicon: chromosome
• Accession: NZ_CP027447
• Organism: Escherichia coli strain 2014C-3075

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: C6W72_RS22370
• Protein ID: WP_096987052.1
• Coordinates: 4356559..4356873 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1NMQ0
• Locus tag: C6W72_RS22365
• Protein ID: WP_000125568.1
• Coordinates: 4356323..4356556 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W72_RS22340	4351397..4351684	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
C6W72_RS22345	4351742..4353073	+	1332	WP_001183201.1	MFS transporter	-
C6W72_RS22350	4353181..4353711	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
C6W72_RS22355	4353704..4355566	+	1863	WP_000377117.1	glutathione-regulated potassium-efflux system protein KefC	-
C6W72_RS22360	4355758..4356237	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
C6W72_RS22365	4356323..4356556	+	234	WP_000125568.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
C6W72_RS22370	4356559..4356873	+	315	WP_096987052.1	CcdB family protein	Toxin
C6W72_RS22375	4356870..4357718	-	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
C6W72_RS22380	4357725..4358102	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
C6W72_RS22385	4358105..4358926	-	822	WP_075861932.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
C6W72_RS22390	4358923..4359912	-	990	WP_088539631.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
C6W72_RS22395	4359912..4361198	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11658.67 Da
• Isoelectric Point: 7.8608

>T98887 WP_096987052.1 NZ_CP027447:4356559-4356873 [Escherichia coli]
MQFTVYCSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T98887 NZ_CP027447:4356559-4356873 [Escherichia coli]
ATGCAATTTACGGTATACTGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.55 Da
• Isoelectric Point: 5.8062

>AT98887 WP_000125568.1 NZ_CP027447:4356323-4356556 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT98887 NZ_CP027447:4356323-4356556 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A829GEF2