Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3996103..3996600 Replicon chromosome
Accession NZ_CP027447
Organism Escherichia coli strain 2014C-3075

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C6W72_RS20570 Protein ID WP_001173343.1
Coordinates 3996427..3996600 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C6W72_RS20565 Protein ID WP_000398619.1
Coordinates 3996103..3996399 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W72_RS20545 3992832..3993551 - 720 WP_000611288.1 two-component system response regulator DcuR -
C6W72_RS20550 3993548..3995179 - 1632 WP_001216448.1 sensor histidine kinase -
C6W72_RS20555 3995360..3995590 + 231 WP_000371713.1 (4Fe-4S)-binding protein -
C6W72_RS20560 3995602..3995874 + 273 WP_000405647.1 N-acetyltransferase -
C6W72_RS20565 3996103..3996399 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C6W72_RS20570 3996427..3996600 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C6W72_RS20575 3996719..3998236 - 1518 WP_000003806.1 lysine--tRNA ligase -
C6W72_RS20580 3998473..3999930 - 1458 WP_000856843.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T98885 WP_001173343.1 NZ_CP027447:3996427-3996600 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T98885 NZ_CP027447:3996427-3996600 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT98885 WP_000398619.1 NZ_CP027447:3996103-3996399 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT98885 NZ_CP027447:3996103-3996399 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References