Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3158675..3159306 Replicon chromosome
Accession NZ_CP027447
Organism Escherichia coli strain 2014C-3075

Toxin (Protein)


Gene name hicA Uniprot ID D8A4M2
Locus tag C6W72_RS16575 Protein ID WP_001259770.1
Coordinates 3159031..3159306 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6W72_RS16570 Protein ID WP_000593555.1
Coordinates 3158675..3159034 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W72_RS16540 3153790..3154263 + 474 WP_096986885.1 PTS sugar transporter subunit IIA -
C6W72_RS16545 3154260..3154541 + 282 WP_000084021.1 PTS sugar transporter subunit IIB -
C6W72_RS16550 3154615..3155973 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
C6W72_RS16555 3155966..3157474 + 1509 WP_096986886.1 carbohydrate kinase -
C6W72_RS16560 3157464..3157733 + 270 WP_000949581.1 HPr family phosphocarrier protein -
C6W72_RS16565 3157765..3158625 + 861 WP_001131792.1 class II aldolase -
C6W72_RS16570 3158675..3159034 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6W72_RS16575 3159031..3159306 - 276 WP_001259770.1 type II toxin-antitoxin system HicA family toxin Toxin
C6W72_RS16580 3159379..3160503 - 1125 WP_001314210.1 ABC transporter permease -
C6W72_RS16585 3160503..3163238 - 2736 WP_096986887.1 ribosome-associated ATPase/putative transporter RbbA -
C6W72_RS16590 3163235..3164302 - 1068 WP_096986888.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3158675..3168141 9466


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10170.90 Da        Isoelectric Point: 10.6128

>T98882 WP_001259770.1 NZ_CP027447:c3159306-3159031 [Escherichia coli]
MRTLVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T98882 NZ_CP027447:c3159306-3159031 [Escherichia coli]
ATGAGAACCCTGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT98882 WP_000593555.1 NZ_CP027447:c3159034-3158675 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT98882 NZ_CP027447:c3159034-3158675 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FQ35

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References