Detailed information of TA system TA098870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 914347..914873
• Replicon: chromosome
• Accession: NZ_CP027447
• Organism: Escherichia coli strain 2014C-3075

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: C6W72_RS04775
• Protein ID: WP_000323025.1
• Coordinates: 914347..914634 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: C6W72_RS04780
• Protein ID: WP_000534858.1
• Coordinates: 914634..914873 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W72_RS04735	909371..909586	-	216	WP_000839590.1	phage lysis protein EssD	-
C6W72_RS27870	909806..909976	+	171	WP_211180497.1	putative zinc-binding protein YnfU	-
C6W72_RS04740	910340..910555	-	216	WP_000066484.1	cold shock-like protein CspB	-
C6W72_RS04745	910856..911068	+	213	WP_000087756.1	cold shock-like protein CspF	-
C6W72_RS27535	911123..911212	+	90	WP_120795389.1	hypothetical protein	-
C6W72_RS04750	911490..912242	-	753	WP_001047135.1	antitermination protein	-
C6W72_RS04755	912256..913305	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
C6W72_RS04760	913307..913585	-	279	WP_012304870.1	hypothetical protein	-
C6W72_RS04765	913652..913903	-	252	WP_000980994.1	hypothetical protein	-
C6W72_RS04770	914120..914275	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
C6W72_RS04775	914347..914634	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
C6W72_RS04780	914634..914873	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
C6W72_RS04785	914898..915203	+	306	WP_001326990.1	hypothetical protein	-
C6W72_RS04790	915406..915738	+	333	WP_001301033.1	protein FlxA	-
C6W72_RS04800	916175..917488	-	1314	WP_000589005.1	ISNCY family transposase	-
C6W72_RS27705	918257..918544	-	288	Protein_907	hypothetical protein	-
C6W72_RS04815	919155..919511	-	357	WP_001310834.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	879785..929221	49436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T98870 WP_000323025.1 NZ_CP027447:c914634-914347 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T98870 NZ_CP027447:c914634-914347 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT98870 WP_000534858.1 NZ_CP027447:c914873-914634 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT98870 NZ_CP027447:c914873-914634 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8