Detailed information of TA system TA098863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 114680..115385
• Replicon: chromosome
• Accession: NZ_CP027447
• Organism: Escherichia coli strain 2014C-3075

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: C6W72_RS00600
• Protein ID: WP_000539521.1
• Coordinates: 114999..115385 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C6W72_RS00595
• Protein ID: WP_001280945.1
• Coordinates: 114680..115009 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W72_RS00580	109838..110749	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
C6W72_RS00585	110926..113274	+	2349	WP_000950331.1	EAL domain-containing protein	-
C6W72_RS00590	113282..114610	+	1329	WP_000086870.1	GGDEF domain-containing protein	-
C6W72_RS00595	114680..115009	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
C6W72_RS00600	114999..115385	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6W72_RS00605	115611..116936	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
C6W72_RS00610	117149..117532	+	384	WP_000497137.1	biofilm formation regulator BssR	-
C6W72_RS00615	117643..118758	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
C6W72_RS00620	118755..119381	-	627	WP_096986484.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T98863 WP_000539521.1 NZ_CP027447:c115385-114999 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T98863 NZ_CP027447:c115385-114999 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT98863 WP_001280945.1 NZ_CP027447:c115009-114680 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT98863 NZ_CP027447:c115009-114680 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6