Detailed information of TA system TA098862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 60096..60655
• Replicon: plasmid unnamed
• Accession: NZ_CP027446
• Organism: Escherichia coli strain 2013C-3492

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C6W68_RS28095
• Protein ID: WP_032206862.1
• Coordinates: 60380..60655 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C6W68_RS28090
• Protein ID: WP_001297026.1
• Coordinates: 60096..60380 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W68_RS28045	55740..55952	+	213	WP_001302189.1	ANR family transcriptional regulator	-
C6W68_RS28050	56197..56658	+	462	WP_047088284.1	thermonuclease family protein	-
C6W68_RS28055	56704..56913	+	210	WP_047088285.1	hemolysin expression modulator Hha	-
C6W68_RS28060	56951..57541	+	591	WP_000766812.1	DUF2726 domain-containing protein	-
C6W68_RS28065	57773..58027	+	255	WP_000083830.1	replication regulatory protein RepA	-
C6W68_RS28075	58263..58337	+	75	WP_001370046.1	RepA leader peptide Tap	-
C6W68_RS28080	58330..59187	+	858	WP_047088286.1	incFII family plasmid replication initiator RepA	-
C6W68_RS28090	60096..60380	+	285	WP_001297026.1	ribbon-helix-helix domain-containing protein	Antitoxin
C6W68_RS28095	60380..60655	+	276	WP_032206862.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6W68_RS28100	60750..60956	+	207	WP_001105064.1	hypothetical protein	-
C6W68_RS28105	61136..62314	-	1179	Protein_65	IS91 family transposase	-
C6W68_RS28110	62314..62679	-	366	WP_000091308.1	hypothetical protein	-
C6W68_RS28115	62716..63860	+	1145	WP_199851309.1	IS3 family transposase	-
C6W68_RS28120	63901..64272	+	372	Protein_68	chromosome partitioning protein ParB	-
C6W68_RS28125	64411..64584	-	174	Protein_69	DUF4113 domain-containing protein	-
C6W68_RS28130	64541..64705	+	165	Protein_70	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papC / hlyC / hlyA / hlyB / hlyD / espP	1..74269	74269

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10680.34 Da
• Isoelectric Point: 9.5302

>T98862 WP_032206862.1 NZ_CP027446:60380-60655 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENS

Nucleotide

• Length: 276 bp

>T98862 NZ_CP027446:60380-60655 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10502.95 Da
• Isoelectric Point: 5.6558

>AT98862 WP_001297026.1 NZ_CP027446:60096-60380 [Escherichia coli]
MQMKNNTVQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT98862 NZ_CP027446:60096-60380 [Escherichia coli]
ATGCAAATGAAAAACAATACCGTACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAAGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA