Detailed information of TA system TA098861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 28847..29382
• Replicon: plasmid unnamed
• Accession: NZ_CP027446
• Organism: Escherichia coli strain 2013C-3492

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C6W68_RS27895
• Protein ID: WP_001270421.1
• Coordinates: 28847..29134 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: C6W68_RS27900
• Protein ID: WP_001132895.1
• Coordinates: 29131..29382 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W68_RS27870	25489..25709	+	221	Protein_22	serine/threonine protein kinase	-
C6W68_RS27875	25830..26570	+	741	WP_032208234.1	site-specific integrase	-
C6W68_RS27880	26813..27790	-	978	WP_000361618.1	RepB family plasmid replication initiator protein	-
C6W68_RS27890	28458..28845	-	388	Protein_25	DDE-type integrase/transposase/recombinase	-
C6W68_RS27895	28847..29134	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6W68_RS27900	29131..29382	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C6W68_RS27905	29556..29741	-	186	WP_032208250.1	hypothetical protein	-
C6W68_RS27910	29906..31083	-	1178	Protein_29	IS91 family transposase	-
C6W68_RS27915	31083..31448	-	366	WP_000091308.1	hypothetical protein	-
C6W68_RS27920	31537..31727	-	191	Protein_31	IS3 family transposase	-
C6W68_RS27925	31987..34174	+	2188	Protein_32	catalase/peroxidase KatP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papC / hlyC / hlyA / hlyB / hlyD / espP	1..74269	74269
	-	inside	IScluster/Tn	-	-	23279..35503	12224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T98861 WP_001270421.1 NZ_CP027446:c29134-28847 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T98861 NZ_CP027446:c29134-28847 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCAGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT98861 WP_001132895.1 NZ_CP027446:c29382-29131 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT98861 NZ_CP027446:c29382-29131 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTGGACTTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6