Detailed information of TA system TA098852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4403820..4404445
• Replicon: chromosome
• Accession: NZ_CP027445
• Organism: Escherichia coli strain 2013C-3492

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6W68_RS23575
• Protein ID: WP_032206177.1
• Coordinates: 4404047..4404445 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6W68_RS23570
• Protein ID: WP_000450524.1
• Coordinates: 4403820..4404047 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W68_RS23545	4399622..4400092	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C6W68_RS23550	4400092..4400664	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6W68_RS23555	4400810..4401688	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6W68_RS23560	4401705..4402739	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C6W68_RS23565	4402952..4403665	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6W68_RS23570	4403820..4404047	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6W68_RS23575	4404047..4404445	+	399	WP_032206177.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6W68_RS23580	4404592..4405455	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
C6W68_RS23585	4405470..4407485	+	2016	WP_032206176.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6W68_RS23590	4407559..4408257	+	699	WP_000679812.1	esterase	-
C6W68_RS23595	4408367..4408567	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14852.17 Da
• Isoelectric Point: 9.2216

>T98852 WP_032206177.1 NZ_CP027445:4404047-4404445 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPGGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T98852 NZ_CP027445:4404047-4404445 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGGTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT98852 WP_000450524.1 NZ_CP027445:4403820-4404047 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT98852 NZ_CP027445:4403820-4404047 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33