Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3942967..3943621 Replicon chromosome
Accession NZ_CP027445
Organism Escherichia coli strain 2013C-3492

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C6W68_RS21220 Protein ID WP_000244777.1
Coordinates 3943214..3943621 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6W68_RS21215 Protein ID WP_000354046.1
Coordinates 3942967..3943233 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W68_RS21190 3938137..3938880 + 744 WP_106888194.1 SDR family oxidoreductase -
C6W68_RS21195 3938937..3940370 - 1434 WP_032207745.1 6-phospho-beta-glucosidase BglA -
C6W68_RS21200 3940415..3940725 + 311 Protein_3854 N(4)-acetylcytidine aminohydrolase -
C6W68_RS21205 3940889..3941548 + 660 WP_000250274.1 hemolysin III family protein -
C6W68_RS21210 3941744..3942724 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C6W68_RS21215 3942967..3943233 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6W68_RS21220 3943214..3943621 + 408 WP_000244777.1 toxin CptA Toxin
C6W68_RS21225 3943661..3944182 - 522 WP_001055874.1 flavodoxin FldB -
C6W68_RS21230 3944294..3945190 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6W68_RS21235 3945214..3945924 + 711 WP_000715227.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6W68_RS21240 3945930..3947662 + 1733 Protein_3862 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T98849 WP_000244777.1 NZ_CP027445:3943214-3943621 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T98849 NZ_CP027445:3943214-3943621 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT98849 WP_000354046.1 NZ_CP027445:3942967-3943233 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT98849 NZ_CP027445:3942967-3943233 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References