Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3297578..3298209 Replicon chromosome
Accession NZ_CP027445
Organism Escherichia coli strain 2013C-3492

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C6W68_RS17665 Protein ID WP_001259385.1
Coordinates 3297578..3297853 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6W68_RS17670 Protein ID WP_000593555.1
Coordinates 3297850..3298209 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W68_RS17650 3292582..3293649 + 1068 WP_032207298.1 HlyD family secretion protein -
C6W68_RS17655 3293646..3296381 + 2736 WP_032207297.1 ribosome-associated ATPase/putative transporter RbbA -
C6W68_RS17660 3296381..3297505 + 1125 WP_001216257.1 ABC transporter permease -
C6W68_RS17665 3297578..3297853 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C6W68_RS17670 3297850..3298209 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6W68_RS17675 3298329..3298730 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C6W68_RS17680 3298736..3299542 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
C6W68_RS17685 3299539..3300303 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
C6W68_RS17690 3300303..3301136 - 834 WP_021539320.1 nickel ABC transporter permease subunit NikC -
C6W68_RS17695 3301133..3302077 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3287308..3298209 10901


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T98846 WP_001259385.1 NZ_CP027445:3297578-3297853 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T98846 NZ_CP027445:3297578-3297853 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT98846 WP_000593555.1 NZ_CP027445:3297850-3298209 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT98846 NZ_CP027445:3297850-3298209 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTATGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References