Detailed information of TA system TA098844

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 2335746..2336449
• Replicon: chromosome
• Accession: NZ_CP027445
• Organism: Escherichia coli strain 2013C-3492

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8T6BCS1
• Locus tag: C6W68_RS12830
• Protein ID: WP_001616568.1
• Coordinates: 2335746..2336087 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: C6W68_RS12835
• Protein ID: WP_032208435.1
• Coordinates: 2336108..2336449 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W68_RS12800	2331161..2332141	+	981	WP_032208436.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
C6W68_RS12805	2332149..2332271	-	123	WP_001300013.1	hypothetical protein	-
C6W68_RS12815	2332663..2333849	-	1187	Protein_2344	IS3-like element IS2 family transposase	-
C6W68_RS12825	2334491..2335498	-	1008	WP_001616572.1	restriction endonuclease	-
C6W68_RS12830	2335746..2336087	-	342	WP_001616568.1	TA system toxin CbtA family protein	Toxin
C6W68_RS12835	2336108..2336449	-	342	WP_032208435.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6W68_RS12840	2336460..2337002	-	543	WP_001616564.1	DNA repair protein RadC	-
C6W68_RS12845	2337015..2337455	-	441	WP_001616563.1	antirestriction protein	-
C6W68_RS12850	2337486..2338307	-	822	WP_032208434.1	DUF945 domain-containing protein	-
C6W68_RS12855	2338405..2338635	-	231	WP_072057388.1	DUF905 domain-containing protein	-
C6W68_RS12860	2338705..2339154	-	450	WP_032208432.1	hypothetical protein	-
C6W68_RS12865	2339151..2339606	-	456	WP_032208430.1	hypothetical protein	-
C6W68_RS12870	2339645..2340280	-	636	WP_001616559.1	hypothetical protein	-
C6W68_RS12875	2340277..2340990	-	714	WP_032208428.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12746.75 Da
• Isoelectric Point: 9.6548

>T98844 WP_001616568.1 NZ_CP027445:c2336087-2335746 [Escherichia coli]
MKTLPATTPQAAKLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T98844 NZ_CP027445:c2336087-2335746 [Escherichia coli]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTAAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12827.46 Da
• Isoelectric Point: 6.4660

>AT98844 WP_032208435.1 NZ_CP027445:c2336449-2336108 [Escherichia coli]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLRLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYYNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT98844 NZ_CP027445:c2336449-2336108 [Escherichia coli]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAGGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACTACAATGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA