Detailed information of TA system TA098842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1747080..1747917
• Replicon: chromosome
• Accession: NZ_CP027445
• Organism: Escherichia coli strain 2013C-3492

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: C6W68_RS09725
• Protein ID: WP_032208386.1
• Coordinates: 1747375..1747917 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: C6W68_RS09720
• Protein ID: WP_001297137.1
• Coordinates: 1747080..1747391 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W68_RS09695	1742100..1743047	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
C6W68_RS09700	1743069..1745060	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
C6W68_RS09705	1745050..1745664	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
C6W68_RS09710	1745664..1745993	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C6W68_RS09715	1746005..1746895	+	891	WP_000971336.1	heme o synthase	-
C6W68_RS09720	1747080..1747391	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
C6W68_RS09725	1747375..1747917	+	543	WP_032208386.1	GNAT family N-acetyltransferase	Toxin
C6W68_RS09730	1747973..1748908	-	936	WP_032208388.1	sel1 repeat family protein	-
C6W68_RS09735	1749316..1750680	+	1365	WP_001000978.1	MFS transporter	-
C6W68_RS09740	1750808..1751299	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
C6W68_RS09745	1751467..1752378	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19943.14 Da
• Isoelectric Point: 8.0422

>T98842 WP_032208386.1 NZ_CP027445:1747375-1747917 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPRQKQFDCGNPVIDKFVHESLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T98842 NZ_CP027445:1747375-1747917 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTAGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACACGAATCACTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT98842 WP_001297137.1 NZ_CP027445:1747080-1747391 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT98842 NZ_CP027445:1747080-1747391 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3