Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 366769..367407 Replicon chromosome
Accession NZ_CP027445
Organism Escherichia coli strain 2013C-3492

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C6W68_RS01855 Protein ID WP_000813794.1
Coordinates 367231..367407 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C6W68_RS01850 Protein ID WP_001270286.1
Coordinates 366769..367185 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W68_RS01830 361921..362862 - 942 WP_032206936.1 ABC transporter permease -
C6W68_RS01835 362863..363876 - 1014 WP_032206935.1 ABC transporter ATP-binding protein -
C6W68_RS01840 363894..365039 - 1146 WP_000047429.1 ABC transporter substrate-binding protein -
C6W68_RS01845 365284..366690 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
C6W68_RS01850 366769..367185 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C6W68_RS01855 367231..367407 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C6W68_RS01860 367629..367859 + 231 WP_071887485.1 YncJ family protein -
C6W68_RS01865 367951..369912 - 1962 Protein_350 23S rRNA 5-hydroxycytidine C2501 synthase -
C6W68_RS01870 369985..370521 - 537 WP_032206933.1 DNA-binding transcriptional regulator SutR -
C6W68_RS01875 370574..371788 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 371828..372976 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T98839 WP_000813794.1 NZ_CP027445:c367407-367231 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T98839 NZ_CP027445:c367407-367231 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGACGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT98839 WP_001270286.1 NZ_CP027445:c367185-366769 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT98839 NZ_CP027445:c367185-366769 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References