Detailed information of TA system TA098836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 10416..11017
• Replicon: plasmid unnamed1
• Accession: NZ_CP027443
• Organism: Escherichia coli strain 2013C-3252

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: MJ69_RS30770
• Protein ID: WP_001216045.1
• Coordinates: 10637..11017 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: MJ69_RS30765
• Protein ID: WP_001190712.1
• Coordinates: 10416..10637 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ69_RS30715	5510..5767	+	258	WP_000382636.1	hypothetical protein	-
MJ69_RS30720	5767..6048	+	282	WP_000002126.1	ASCH domain-containing protein	-
MJ69_RS30725	6072..6365	+	294	WP_000267998.1	hypothetical protein	-
MJ69_RS30730	6372..6746	+	375	WP_000988652.1	hypothetical protein	-
MJ69_RS30735	6728..7403	+	676	Protein_18	hypothetical protein	-
MJ69_RS30740	7400..7762	+	363	WP_001261541.1	hypothetical protein	-
MJ69_RS30750	8838..9194	-	357	WP_001062547.1	hypothetical protein	-
MJ69_RS30755	9195..9779	-	585	WP_001133673.1	hypothetical protein	-
MJ69_RS30760	9954..10343	+	390	WP_000506724.1	DNA repair protein	-
MJ69_RS30765	10416..10637	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MJ69_RS30770	10637..11017	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MJ69_RS30775	11022..11201	+	180	WP_000113018.1	hypothetical protein	-
MJ69_RS30780	11229..12272	+	1044	WP_000648833.1	DUF968 domain-containing protein	-
MJ69_RS30785	12361..12813	+	453	WP_001326849.1	late promoter-activating protein	-
MJ69_RS30790	12899..14092	+	1194	WP_097463274.1	terminase	-
MJ69_RS30795	14092..15576	+	1485	WP_000124153.1	hypothetical protein	-
MJ69_RS32475	15681..15911	+	231	Protein_30	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95157	95157

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T98836 WP_001216045.1 NZ_CP027443:10637-11017 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T98836 NZ_CP027443:10637-11017 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT98836 WP_001190712.1 NZ_CP027443:10416-10637 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT98836 NZ_CP027443:10416-10637 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6