Detailed information of TA system TA098829

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4100321..4101153
• Replicon: chromosome
• Accession: NZ_CP027442
• Organism: Escherichia coli strain 2013C-3252

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: MJ69_RS22495
• Protein ID: WP_000854753.1
• Coordinates: 4100779..4101153 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: MJ69_RS22490
• Protein ID: WP_001315620.1
• Coordinates: 4100321..4100689 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ69_RS22455	4097157..4097612	+	456	WP_000581504.1	hypothetical protein	-
MJ69_RS22460	4097691..4097924	+	234	WP_001119729.1	DUF905 family protein	-
MJ69_RS22470	4098024..4098842	+	819	WP_001234622.1	DUF945 domain-containing protein	-
MJ69_RS22475	4098897..4099382	+	486	WP_000849588.1	antirestriction protein	-
MJ69_RS22480	4099398..4099874	+	477	WP_001186774.1	RadC family protein	-
MJ69_RS22485	4099937..4100158	+	222	WP_000692345.1	DUF987 domain-containing protein	-
MJ69_RS22490	4100321..4100689	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MJ69_RS22495	4100779..4101153	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
MJ69_RS22500	4101150..4101638	+	489	WP_000777547.1	hypothetical protein	-
MJ69_RS22505	4101650..4101847	+	198	WP_000839281.1	DUF957 domain-containing protein	-
MJ69_RS32430	4101932..4102084	+	153	WP_001280445.1	hypothetical protein	-
MJ69_RS22530	4103129..4103779	+	651	WP_001037966.1	HNH endonuclease	-
MJ69_RS22535	4103787..4104767	-	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
MJ69_RS22540	4104832..4105938	-	1107	WP_001341302.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	4041064..4109806	68742

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T98829 WP_000854753.1 NZ_CP027442:4100779-4101153 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T98829 NZ_CP027442:4100779-4101153 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT98829 WP_001315620.1 NZ_CP027442:4100321-4100689 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT98829 NZ_CP027442:4100321-4100689 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5