Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3838678..3839175 Replicon chromosome
Accession NZ_CP027442
Organism Escherichia coli strain 2013C-3252

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MJ69_RS21070 Protein ID WP_001173343.1
Coordinates 3839002..3839175 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MJ69_RS21065 Protein ID WP_000398619.1
Coordinates 3838678..3838974 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ69_RS21040 3835409..3836128 - 720 WP_000611288.1 two-component system response regulator DcuR -
MJ69_RS21045 3836125..3837756 - 1632 WP_001216487.1 sensor histidine kinase -
MJ69_RS21050 3837937..3838167 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
MJ69_RS21055 3838179..3838451 + 273 WP_000405647.1 N-acetyltransferase -
MJ69_RS21065 3838678..3838974 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MJ69_RS21070 3839002..3839175 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MJ69_RS21075 3839294..3840811 - 1518 WP_001295074.1 lysine--tRNA ligase -
MJ69_RS21080 3841048..3842603 - 1556 Protein_3845 oligopeptide:H+ symporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T98827 WP_001173343.1 NZ_CP027442:3839002-3839175 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T98827 NZ_CP027442:3839002-3839175 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT98827 WP_000398619.1 NZ_CP027442:3838678-3838974 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT98827 NZ_CP027442:3838678-3838974 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References