Detailed information of TA system TA098824

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2481297..2481951
• Replicon: chromosome
• Accession: NZ_CP027442
• Organism: Escherichia coli strain 2013C-3252

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: MJ69_RS14065
• Protein ID: WP_000244777.1
• Coordinates: 2481297..2481704 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: MJ69_RS14070
• Protein ID: WP_000354043.1
• Coordinates: 2481685..2481951 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ69_RS14045	2477254..2478987	-	1734	WP_000813220.1	single-stranded-DNA-specific exonuclease RecJ	-
MJ69_RS14050	2478993..2479703	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
MJ69_RS14055	2479728..2480624	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
MJ69_RS14060	2480736..2481257	+	522	WP_001055874.1	flavodoxin FldB	-
MJ69_RS14065	2481297..2481704	-	408	WP_000244777.1	toxin CptA	Toxin
MJ69_RS14070	2481685..2481951	-	267	WP_000354043.1	FAD assembly factor SdhE	Antitoxin
MJ69_RS14075	2482194..2483174	+	981	WP_000886095.1	tRNA-modifying protein YgfZ	-
MJ69_RS14080	2483370..2484029	-	660	WP_000250274.1	hemolysin III family protein	-
MJ69_RS14085	2484193..2484504	-	312	WP_001182954.1	N(4)-acetylcytidine aminohydrolase	-
MJ69_RS14090	2484549..2485982	+	1434	WP_158707522.1	6-phospho-beta-glucosidase BglA	-
MJ69_RS14095	2486039..2486782	-	744	WP_000951964.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T98824 WP_000244777.1 NZ_CP027442:c2481704-2481297 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T98824 NZ_CP027442:c2481704-2481297 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10563.16 Da
• Isoelectric Point: 5.1599

>AT98824 WP_000354043.1 NZ_CP027442:c2481951-2481685 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDLDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT98824 NZ_CP027442:c2481951-2481685 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCTGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7