Detailed information of TA system TA098821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1886490..1887115
• Replicon: chromosome
• Accession: NZ_CP027442
• Organism: Escherichia coli strain 2013C-3252

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MJ69_RS10810
• Protein ID: WP_000911330.1
• Coordinates: 1886490..1886888 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: MJ69_RS10815
• Protein ID: WP_000450524.1
• Coordinates: 1886888..1887115 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ69_RS10790	1882368..1882568	+	201	WP_000383836.1	YpfN family protein	-
MJ69_RS10795	1882678..1883376	-	699	WP_000679812.1	esterase	-
MJ69_RS10800	1883450..1885465	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
MJ69_RS10805	1885480..1886343	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
MJ69_RS10810	1886490..1886888	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MJ69_RS10815	1886888..1887115	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
MJ69_RS10820	1887269..1887982	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
MJ69_RS10825	1888195..1889229	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
MJ69_RS10830	1889246..1890124	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MJ69_RS10835	1890270..1890842	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
MJ69_RS10840	1890842..1891312	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T98821 WP_000911330.1 NZ_CP027442:c1886888-1886490 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T98821 NZ_CP027442:c1886888-1886490 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT98821 WP_000450524.1 NZ_CP027442:c1887115-1886888 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT98821 NZ_CP027442:c1887115-1886888 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33