Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1057310..1057781 Replicon chromosome
Accession NZ_CP027442
Organism Escherichia coli strain 2013C-3252

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag MJ69_RS06470 Protein ID WP_001303511.1
Coordinates 1057503..1057781 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag MJ69_RS06465 Protein ID WP_001302048.1
Coordinates 1057310..1057501 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ69_RS06415 1052388..1052606 - 219 WP_000683609.1 DUF4014 family protein -
MJ69_RS06420 1052734..1053045 - 312 WP_001289673.1 hypothetical protein -
MJ69_RS06425 1053038..1053265 - 228 WP_000699809.1 hypothetical protein -
MJ69_RS06430 1053262..1053543 - 282 WP_000603384.1 DNA-binding protein -
MJ69_RS06435 1053576..1054337 - 762 WP_000450872.1 DUF1627 domain-containing protein -
MJ69_RS06440 1054363..1055104 - 742 Protein_1184 ATP-binding protein -
MJ69_RS06445 1055111..1056073 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
MJ69_RS06450 1056096..1056521 - 426 WP_000693943.1 Rha family transcriptional regulator -
MJ69_RS06455 1056518..1056820 - 303 WP_000172738.1 transcriptional regulator -
MJ69_RS06460 1056918..1057289 + 372 WP_001169687.1 hypothetical protein -
MJ69_RS06465 1057310..1057501 + 192 WP_001302048.1 hypothetical protein Antitoxin
MJ69_RS06470 1057503..1057781 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MJ69_RS06475 1058068..1058220 + 153 WP_001427316.1 DUF1391 family protein -
MJ69_RS06480 1058641..1058862 + 222 WP_000560218.1 killing protein KilR -
MJ69_RS06485 1058856..1059032 + 177 WP_000245534.1 hypothetical protein -
MJ69_RS06490 1059106..1059381 + 276 WP_001307773.1 hypothetical protein -
MJ69_RS06495 1059480..1062131 + 2652 WP_000105101.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 971092..1072570 101478


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T98811 WP_001303511.1 NZ_CP027442:1057503-1057781 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T98811 NZ_CP027442:1057503-1057781 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98811 WP_001302048.1 NZ_CP027442:1057310-1057501 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98811 NZ_CP027442:1057310-1057501 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References