Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
Location | 31765..32290 | Replicon | plasmid unnamed |
Accession | NZ_CP027441 | ||
Organism | Escherichia coli strain 2012C-4502 |
Toxin (Protein)
Gene name | ccdB | Uniprot ID | S1PFV8 |
Locus tag | C6W69_RS25560 | Protein ID | WP_001159871.1 |
Coordinates | 31765..32070 (-) | Length | 102 a.a. |
Antitoxin (Protein)
Gene name | ccdA | Uniprot ID | Q3ZU16 |
Locus tag | C6W69_RS25565 | Protein ID | WP_000813639.1 |
Coordinates | 32072..32290 (-) | Length | 73 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
C6W69_RS25530 | 27760..27933 | + | 174 | Protein_37 | DUF4113 domain-containing protein | - |
C6W69_RS25535 | 27935..28351 | - | 417 | WP_001278809.1 | plasmid partitioning/stability family protein | - |
C6W69_RS25540 | 28344..29324 | - | 981 | WP_000688510.1 | plasmid segregation protein ParM | - |
C6W69_RS25545 | 29739..30047 | - | 309 | WP_000030199.1 | hypothetical protein | - |
C6W69_RS25550 | 30134..30778 | - | 645 | WP_001144036.1 | ParA family protein | - |
C6W69_RS25555 | 30958..31764 | - | 807 | WP_000016966.1 | site-specific integrase | - |
C6W69_RS25560 | 31765..32070 | - | 306 | WP_001159871.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
C6W69_RS25565 | 32072..32290 | - | 219 | WP_000813639.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
C6W69_RS25570 | 32886..33146 | + | 261 | WP_000343100.1 | hypothetical protein | - |
C6W69_RS25575 | 33143..33733 | + | 591 | WP_106905472.1 | hypothetical protein | - |
C6W69_RS25580 | 33751..34098 | - | 348 | WP_000142427.1 | hypothetical protein | - |
C6W69_RS25590 | 34581..34952 | - | 372 | WP_001373114.1 | colicin | - |
C6W69_RS25595 | 35012..35743 | - | 732 | WP_000850414.1 | complement resistance protein TraT | - |
C6W69_RS25605 | 36015..36125 | - | 111 | Protein_50 | IS481 family transposase | - |
C6W69_RS26730 | 36129..36346 | - | 218 | Protein_51 | hypothetical protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Conjugative plasmid | - | hlyD / hlyB / hlyA / hlyC / espP | 1..173714 | 173714 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11692.49 Da Isoelectric Point: 6.4674
>T98809 WP_001159871.1 NZ_CP027441:c32070-31765 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T98809 NZ_CP027441:c32070-31765 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8372.30 Da Isoelectric Point: 4.5203
>AT98809 WP_000813639.1 NZ_CP027441:c32290-32072 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT98809 NZ_CP027441:c32290-32072 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A829CCE8 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7U9DIR5 |