Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 31765..32290 Replicon plasmid unnamed
Accession NZ_CP027441
Organism Escherichia coli strain 2012C-4502

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C6W69_RS25560 Protein ID WP_001159871.1
Coordinates 31765..32070 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C6W69_RS25565 Protein ID WP_000813639.1
Coordinates 32072..32290 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W69_RS25530 27760..27933 + 174 Protein_37 DUF4113 domain-containing protein -
C6W69_RS25535 27935..28351 - 417 WP_001278809.1 plasmid partitioning/stability family protein -
C6W69_RS25540 28344..29324 - 981 WP_000688510.1 plasmid segregation protein ParM -
C6W69_RS25545 29739..30047 - 309 WP_000030199.1 hypothetical protein -
C6W69_RS25550 30134..30778 - 645 WP_001144036.1 ParA family protein -
C6W69_RS25555 30958..31764 - 807 WP_000016966.1 site-specific integrase -
C6W69_RS25560 31765..32070 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C6W69_RS25565 32072..32290 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6W69_RS25570 32886..33146 + 261 WP_000343100.1 hypothetical protein -
C6W69_RS25575 33143..33733 + 591 WP_106905472.1 hypothetical protein -
C6W69_RS25580 33751..34098 - 348 WP_000142427.1 hypothetical protein -
C6W69_RS25590 34581..34952 - 372 WP_001373114.1 colicin -
C6W69_RS25595 35012..35743 - 732 WP_000850414.1 complement resistance protein TraT -
C6W69_RS25605 36015..36125 - 111 Protein_50 IS481 family transposase -
C6W69_RS26730 36129..36346 - 218 Protein_51 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..173714 173714


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T98809 WP_001159871.1 NZ_CP027441:c32070-31765 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T98809 NZ_CP027441:c32070-31765 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT98809 WP_000813639.1 NZ_CP027441:c32290-32072 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT98809 NZ_CP027441:c32290-32072 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References