Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4883239..4884023 Replicon chromosome
Accession NZ_CP027440
Organism Escherichia coli strain 2012C-4502

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag C6W69_RS25260 Protein ID WP_000613626.1
Coordinates 4883239..4883733 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag C6W69_RS25265 Protein ID WP_001110446.1
Coordinates 4883730..4884023 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W69_RS25240 4878432..4879529 + 1098 WP_000589326.1 flagellar basal body P-ring protein FlgI -
C6W69_RS25245 4879529..4880470 + 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
C6W69_RS25250 4880536..4882179 + 1644 WP_042105957.1 flagellar hook-associated protein FlgK -
C6W69_RS25255 4882191..4883144 + 954 WP_001212762.1 flagellar hook-associated protein FlgL -
C6W69_RS25260 4883239..4883733 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
C6W69_RS25265 4883730..4884023 - 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
C6W69_RS25270 4884157..4887342 - 3186 WP_088144504.1 ribonuclease E -
C6W69_RS25280 4887915..4888874 + 960 WP_001563107.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T98808 WP_000613626.1 NZ_CP027440:c4883733-4883239 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T98808 NZ_CP027440:c4883733-4883239 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT98808 WP_001110446.1 NZ_CP027440:c4884023-4883730 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT98808 NZ_CP027440:c4884023-4883730 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTATCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References