Detailed information of TA system TA098807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4630970..4631675
• Replicon: chromosome
• Accession: NZ_CP027440
• Organism: Escherichia coli strain 2012C-4502

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1Q4PEL5
• Locus tag: C6W69_RS23985
• Protein ID: WP_001562827.1
• Coordinates: 4631289..4631675 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C6W69_RS23980
• Protein ID: WP_001280945.1
• Coordinates: 4630970..4631299 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W69_RS23965	4626128..4627039	+	912	WP_001538499.1	glutathione ABC transporter permease GsiD	-
C6W69_RS23970	4627216..4629564	+	2349	WP_106905419.1	EAL domain-containing protein	-
C6W69_RS23975	4629572..4630900	+	1329	WP_001562826.1	GGDEF domain-containing protein	-
C6W69_RS23980	4630970..4631299	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
C6W69_RS23985	4631289..4631675	-	387	WP_001562827.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6W69_RS23990	4631901..4633226	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
C6W69_RS23995	4633439..4633822	+	384	WP_000497137.1	biofilm formation regulator BssR	-
C6W69_RS24000	4633933..4635048	+	1116	WP_001562828.1	aldose sugar dehydrogenase YliI	-
C6W69_RS24005	4635045..4635671	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14275.42 Da
• Isoelectric Point: 9.9296

>T98807 WP_001562827.1 NZ_CP027440:c4631675-4631289 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVIQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T98807 NZ_CP027440:c4631675-4631289 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATTCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT98807 WP_001280945.1 NZ_CP027440:c4631299-4630970 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT98807 NZ_CP027440:c4631299-4630970 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1Q4PEL5