Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4233025..4233643 Replicon chromosome
Accession NZ_CP027440
Organism Escherichia coli strain 2012C-4502

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C6W69_RS21975 Protein ID WP_001291435.1
Coordinates 4233025..4233243 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C6W69_RS21980 Protein ID WP_000344800.1
Coordinates 4233269..4233643 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W69_RS21940 4228316..4228885 + 570 WP_001561544.1 YbaY family lipoprotein -
C6W69_RS21945 4228916..4229227 - 312 WP_000409911.1 MGMT family protein -
C6W69_RS21955 4229606..4229959 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C6W69_RS21960 4230001..4231551 - 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C6W69_RS21965 4231715..4232185 - 471 WP_000136192.1 YlaC family protein -
C6W69_RS21970 4232301..4232852 - 552 WP_000102539.1 maltose O-acetyltransferase -
C6W69_RS21975 4233025..4233243 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C6W69_RS21980 4233269..4233643 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C6W69_RS21985 4234189..4237338 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C6W69_RS21990 4237361..4238554 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T98805 WP_001291435.1 NZ_CP027440:c4233243-4233025 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T98805 NZ_CP027440:c4233243-4233025 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT98805 WP_000344800.1 NZ_CP027440:c4233643-4233269 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT98805 NZ_CP027440:c4233643-4233269 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References