Detailed information of TA system TA098802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2595093..2595688
• Replicon: chromosome
• Accession: NZ_CP027440
• Organism: Escherichia coli strain 2012C-4502

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D6JG31
• Locus tag: C6W69_RS13630
• Protein ID: WP_000155159.1
• Coordinates: 2595093..2595470 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: C6W69_RS13635
• Protein ID: WP_000557315.1
• Coordinates: 2595467..2595688 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W69_RS13610	2590591..2591661	-	1071	WP_001562225.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-
C6W69_RS13615	2591663..2592508	-	846	WP_000572164.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
C6W69_RS13620	2592505..2593392	-	888	WP_000099289.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
C6W69_RS13625	2593568..2594884	-	1317	WP_000803191.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
C6W69_RS13630	2595093..2595470	-	378	WP_000155159.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C6W69_RS13635	2595467..2595688	-	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C6W69_RS13640	2595776..2596489	-	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
C6W69_RS13645	2596491..2597258	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
C6W69_RS13650	2597255..2598532	-	1278	WP_106905274.1	branched chain amino acid ABC transporter permease LivM	-
C6W69_RS13655	2598529..2599455	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
C6W69_RS13660	2599503..2600612	-	1110	WP_001316697.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2589851..2598532	8681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.25 Da
• Isoelectric Point: 6.7263

>T98802 WP_000155159.1 NZ_CP027440:c2595470-2595093 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T98802 NZ_CP027440:c2595470-2595093 [Escherichia coli]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGCTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT98802 WP_000557315.1 NZ_CP027440:c2595688-2595467 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT98802 NZ_CP027440:c2595688-2595467 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTAGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGTCATAAGGCAGCTGTGATTATCAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: D6JG31

Antitoxin

• AlphaFold DB: D6JG32