Detailed information of TA system TA098788

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 461761..462397
• Replicon: chromosome
• Accession: NZ_CP027440
• Organism: Escherichia coli strain 2012C-4502

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: C6W69_RS02665
• Protein ID: WP_000813796.1
• Coordinates: 461761..461937 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C6W69_RS02670
• Protein ID: WP_076838470.1
• Coordinates: 461981..462397 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W69_RS02645	457383..458594	-	1212	WP_071602029.1	BenE family transporter YdcO	-
C6W69_RS02650	458647..459183	+	537	WP_001563332.1	DNA-binding transcriptional regulator SutR	-
C6W69_RS02655	459256..461217	+	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C6W69_RS02660	461309..461539	-	231	WP_024196315.1	DUF2554 family protein	-
C6W69_RS02665	461761..461937	+	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C6W69_RS02670	461981..462397	+	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C6W69_RS02675	462476..463882	+	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
C6W69_RS02680	464127..465272	+	1146	WP_001563335.1	ABC transporter substrate-binding protein	-
C6W69_RS02685	465290..466303	+	1014	WP_001563336.1	ABC transporter ATP-binding protein	-
C6W69_RS02690	466304..467245	+	942	WP_001563337.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T98788 WP_000813796.1 NZ_CP027440:461761-461937 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T98788 NZ_CP027440:461761-461937 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT98788 WP_076838470.1 NZ_CP027440:461981-462397 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT98788 NZ_CP027440:461981-462397 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3