Detailed information of TA system TA098774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 56595..57120
• Replicon: plasmid unnamed1
• Accession: NZ_CP027438
• Organism: Escherichia coli strain 2012C-4221

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: C6W65_RS25865
• Protein ID: WP_106907027.1
• Coordinates: 56595..56900 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: C6W65_RS25870
• Protein ID: WP_000813639.1
• Coordinates: 56902..57120 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W65_RS25830	51695..52087	-	393	WP_000272016.1	plasmid partitioning/stability family protein	-
C6W65_RS25835	52091..53065	-	975	WP_097469860.1	StbA family protein	-
C6W65_RS25840	53304..53678	-	375	WP_106907023.1	hypothetical protein	-
C6W65_RS25845	53678..54310	-	633	WP_000312330.1	ParA family protein	-
C6W65_RS25855	55066..55821	-	756	WP_000807692.1	replication initiation protein RepE	-
C6W65_RS25860	56324..56594	-	271	Protein_73	tyrosine-type recombinase/integrase	-
C6W65_RS25865	56595..56900	-	306	WP_106907027.1	type II toxin-antitoxin system toxin CcdB	Toxin
C6W65_RS25870	56902..57120	-	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
C6W65_RS25875	57513..57884	-	372	Protein_76	Tn3 family transposase	-
C6W65_RS25880	58004..58378	-	375	Protein_77	transposase zinc-binding domain-containing protein	-
C6W65_RS25885	58333..58581	-	249	WP_158709094.1	hypothetical protein	-
C6W65_RS27075	58727..58983	+	257	Protein_79	IS3 family transposase	-
C6W65_RS25895	59234..59941	-	708	WP_106907045.1	AraC family transcriptional regulator	-
C6W65_RS25900	60978..61385	+	408	WP_158709095.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..74904	74904

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11726.50 Da
• Isoelectric Point: 6.4674

>T98774 WP_106907027.1 NZ_CP027438:c56900-56595 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADFSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T98774 NZ_CP027438:c56900-56595 [Escherichia coli]
ATGCAGTTTAAAGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGACGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTAGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATTTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT98774 WP_000813639.1 NZ_CP027438:c57120-56902 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT98774 NZ_CP027438:c57120-56902 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9DIR5