Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 29733..30268 Replicon plasmid unnamed1
Accession NZ_CP027438
Organism Escherichia coli strain 2012C-4221

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag C6W65_RS25630 Protein ID WP_000222766.1
Coordinates 29733..30020 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag C6W65_RS25635 Protein ID WP_001132900.1
Coordinates 30017..30268 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W65_RS25595 24984..25574 + 591 WP_106906987.1 hypothetical protein -
C6W65_RS25600 25594..25941 - 348 WP_106906989.1 hypothetical protein -
C6W65_RS25610 26070..26405 + 336 Protein_33 colicin E1 immunity protein -
C6W65_RS25615 26427..28306 - 1880 Protein_34 colicin -
C6W65_RS25625 29159..29563 - 405 WP_000154256.1 DUF2251 domain-containing protein -
C6W65_RS25630 29733..30020 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6W65_RS25635 30017..30268 - 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
C6W65_RS25650 31231..32088 - 858 WP_158709091.1 incFII family plasmid replication initiator RepA -
C6W65_RS25655 32081..32155 - 75 WP_001375168.1 RepA leader peptide Tap -
C6W65_RS25665 32386..32643 - 258 WP_106906995.1 replication regulatory protein RepA -
C6W65_RS25670 32927..33076 - 150 WP_106906997.1 type I toxin-antitoxin system Hok family toxin -
- 33121..33180 + 60 NuclAT_0 - -
- 33121..33180 + 60 NuclAT_0 - -
- 33121..33180 + 60 NuclAT_0 - -
- 33121..33180 + 60 NuclAT_0 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..74904 74904


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T98769 WP_000222766.1 NZ_CP027438:c30020-29733 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T98769 NZ_CP027438:c30020-29733 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT98769 WP_001132900.1 NZ_CP027438:c30268-30017 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT98769 NZ_CP027438:c30268-30017 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAGCGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References