Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3953820..3954474 Replicon chromosome
Accession NZ_CP027437
Organism Escherichia coli strain 2012C-4221

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag C6W65_RS20250 Protein ID WP_000244781.1
Coordinates 3954067..3954474 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6W65_RS20245 Protein ID WP_000354046.1
Coordinates 3953820..3954086 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W65_RS20225 3949908..3951341 - 1434 WP_025210839.1 6-phospho-beta-glucosidase BglA -
C6W65_RS20230 3951386..3951697 + 312 WP_106906634.1 N(4)-acetylcytidine aminohydrolase -
C6W65_RS20235 3951861..3952520 + 660 WP_000250274.1 hemolysin III family protein -
C6W65_RS20240 3952597..3953577 - 981 WP_106906635.1 tRNA-modifying protein YgfZ -
C6W65_RS20245 3953820..3954086 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6W65_RS20250 3954067..3954474 + 408 WP_000244781.1 protein YgfX Toxin
C6W65_RS20255 3954514..3955035 - 522 WP_001055872.1 flavodoxin FldB -
C6W65_RS20260 3955147..3956043 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6W65_RS20265 3956068..3956778 + 711 WP_072648287.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6W65_RS20270 3956784..3958517 + 1734 WP_106906636.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T98765 WP_000244781.1 NZ_CP027437:3954067-3954474 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T98765 NZ_CP027437:3954067-3954474 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGTGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT98765 WP_000354046.1 NZ_CP027437:3953820-3954086 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT98765 NZ_CP027437:3953820-3954086 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References